Here we provide a description of less frequently used workflows.

• Split multi-channel images to obain single-channel images for analysis.
• Create numbered label images to create an image with color-code segmentation masks including their identifer.
• Calculate distance to objects to calculate images summarizing which object is closest to each pixel.
• Convert segmentation results in FQ outlines that can be used by the older Matlab version of FISH-quant.

Each workflow is documented in a dedicated section.